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. 2020 Oct 28;21(12):e51252. doi: 10.15252/embr.202051252

Figure 3. SARS‐CoV‐2 challenge does not affect the IFN response in alveolar macrophages.

Figure 3

  • A–E
    (A) Timeline of the experiment. (B) Total RNA was harvested and subjected to qPCR for quantification of the IFN response measured by IFNA2, IFNB1 and IFNL1. (C) ISG response measured by ISG15, RSAD2 and IFIT1 (Donors marked in green and red were not quantifiable in all test conditions). (D) Quantification of the presence of SARS‐CoV‐2. (E) Western blots were performed using samples from two donors. (B–D) Each colour corresponds to a donor; 5 different donors were analysed. The dashed line represents the detection limit. An ordinary one‐way ANOVA test was used for statistical analysis: ns, not significant; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

Alveolar macrophages from different donors were purified from BAL fluid and infected with SARS‐CoV‐2 before treatment with either Poly(I:C) or IFN‐λ1 (1 ng/ml).

Source data are available online for this figure.
 Source data are available online for this figure.