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. 2020 Oct 28;21(12):e51252. doi: 10.15252/embr.202051252

Figure 5. Determination of viral infection effectivity.

Figure 5

  1. Total RNA was harvested from AMs and primary human airway epithelial cells (HAE) and subjected to qPCR for quantification of the ACE2 receptor. Each colour corresponds to a donor. The dashed line represents the detection limit.
  2. The presence of nucleoprotein mRNA of SARS‐CoV‐2 in AMs and primary HAE was also quantified after SARS‐CoV‐2 treatment. (A–B) Each colour corresponds to a donor. An unpaired t‐test test was used for statistical analysis: ****P ≤ 0.0001.
  3. Primer efficiency was tested using serial dilutions of positive samples using qPCR. The C t values were plotted on a logarithmic scale along with the corresponding dilutions before performing a linear regression in order to calculate the slope of the trend line through the data points. This analysis revealed an amplification factor of 1.92, 1.91 and 1.85 for the SARS‐CoV‐2, IAV and SeV primer set, respectively. The different colours correspond to different primer sets.

Source data are available online for this figure.
 Source data are available online for this figure.