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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Thromb Haemost. 2020 Mar 30;18(6):1278–1290. doi: 10.1111/jth.14785

Figure 2. The effect of cysteine mutagenesis on the clearance of A1A2A3-VWF.

Figure 2.

(A) All novel A1A2A3-VWF cysteine variants were secreted and purified from HEK293F cells. (B, C and D) Clearance of non-PEGylated A1A2A3 cysteine variants were examined in VWF−/− mice, with 3-4 mice per time point. (E) The mean resident time (MRT) of all non-PEGylated variants was calculated using one phase decay fit and graphed as ± SEM. (* p <0.05, ** p <0.01, respectively).