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The PEGylation efficacy for each of the A1A2A3 variants as measured by analytical size exchange chromatography following PEG maleimide conjugation and purification. No conjugation of PEG to WT-A1A2A3 was observed.
| A1A2A3 Variant | % PEGylated species |
|---|---|
| WT A1A2A3 | 0 |
| S1286C | 99.3 |
| Q1353C | 90.5 |
| M1545C | 94.7 |
| L1591C | 96.1 |
| V1636C | 79.3 |
| Q1652C | 91.1 |
| V1803C | 89.8 |
| S1807C | 94.7 |
