Figure 2.

LSD1 inhibition enhances the ex vivo propagation of long-term repopulating HSCs. (A) Colony-forming capacity of CD34⁺ cells expanded with 2-PCPA or UM171 for 6 days. Accumulated data from 2 independent experiments with 3 replicates each. hCD45 engraftment in peripheral blood at week 5 (B) and hCD45 engraftment in BM at week 16 (C) in NSG mice transplanted with the progeny of 20 000 plated UCB CD34⁺ cells expanded with DMSO, 2-PCPA, or UM171 for 6 days. Five mice per each group was used as starting number. (D) Plot summarizing data obtained from LDA analysis. (E) Total number of SRCs obtained from LDA of cultures treated with DMSO, 2-PCPA, or UM171. Numbers are normalized to 1 million CD34⁺ cells before culture. *P ≤ .05, **P ≤ .01, generalized linear models.