Figure 4.

GRB7 promotes the cell cycle G1/S transition in bladder cancer cells. A. Flow cytometric analysis of stable cell lines constructed from 5637 and RT4 cells demonstrated that the percentage of S phase cells negatively correlated with the expression of TEF. B. Representative micrographs of BrdU showed that the percentage of BrdU-incorporated cells were higher in GRB7-overexpressed cells but lower in GRB7-silenced cells. C. the quantification of BrdU incorporation in stable cell lines. Three independent experiments were conducted. D. Western blot analysis of G1/S transition-associated genes showed that the protein expression levels of the cell cycle promoter is positively expressed with GRB7 expression, while the protein expression levels of the cell cycle inhibitor is negatively expressed with GRB7 expression. *P < 0.05.