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. 2020 Oct 19;16(16):3184–3199. doi: 10.7150/ijbs.45505

Figure 2.

Figure 2

MPT0B291 reduces the proliferation of U-87MG and C6 cells but not in the human astrocytes. The U-87MG human glioma cells versus human hippocampal astrocytes (HA-h) and C6 rat glioma cells versus neuron/glia mixed primary culture were used, and cell viability was measured using the MTT assay at 24 in U-87MG (A), HA-h (B), C6 cells (C) and primary culture (N/G) (D). (E) Representative images of immunofluorescence (IF) staining for Ki67 on the C6 and neuron/glia primary culture cells treated with vehicle (Veh), MPT0B291 (30 µM), SAHA (30 µM) and TMZ (30 µM) for 24 h. The Ki67 is shown in red and GFAP is shown in green. (F) Representative images of immunocytochemistry (ICC) for Ki67 on the U-87MG cells. The Ki67 is shown in brown (Calibration = 50 µm). (G) The percentage of Ki67-positive cells is normalized with total C6 cell number and presented as mean ± SEM (n=4 in each group) *p<0.05 versus the vehicle-treated group.