Skip to main content
. 2020 Oct 19;16(16):3184–3199. doi: 10.7150/ijbs.45505

Figure 4.

Figure 4

MPT0B291 induces cell death and apoptosis in glioma cells in vitro and in vivo. The LDH activity in the medium of U-87MG (A), HA-h (B), C6 (C), and neuron/glia mixed primary culture (N/G) (D) treated with various concentrations of MPT0B291, SAHA and TMZ was measured and data are presented as mean ± SEM. (n=3 in each group) *p<0.05 versus vehicle-treated group. (E) Representative images of IF for cleaved caspase-3 in the C6 cells and neuron/glia primary culture treated with vehicle (Veh), MPT0B291 (30μM), SAHA (30μM) and TMZ (30 µM) for 24 h. (Calibration = 50 µm). (F) The percentage of cleaved caspase-3 positive cells is normalized with total C6 cell number and presented as mean ± SEM (n=4 in each group) *p<0.05 versus the vehicle-treated group. (G) Representative images of HE-stained brain sections from sham and allografted animals with or without MPT0B291 treatment. Arrow in the inset indicated apoptotic bodies, which is characteristics of apoptotic cells (Calibration = 50 µm).