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. 2020 Oct 13;59(1):e126. doi: 10.1002/cpmc.126

Figure 2.

Figure 2

Schematic representation of the pBAC plasmid to rescue rSARS‐CoV‐2: The full‐length cDNA of the SARS‐CoV‐2 genome is flanked at the 5′ end by the cytomegalovirus (CMV) polymerase II−driven promoter and at the 3′ end by the hepatitis delta ribozyme (Rz) and bovine growth hormone (bGH) polyadenylation signal. The entire ∼30,815‐bp construct was inserted into the pBeloBAC11 plasmid using Pcil and HindIII restriction sites. The pBeloBAC11 plasmid is an E. coli vector commonly used to generate BACs because it can support the insertion of large DNA fragments as a single copy in cells. The pBeloBAC11 contains a chloramphenicol resistance (CmR) gene as a selective marker. Viral proteins are those previously described in Figure 1.