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. 2020 Aug 12;18(2):104–119. doi: 10.1016/j.gpb.2019.11.008

Figure 1.

Figure 1

Workflow for building DPHL

A. Schematic representation of DDA shotgun proteomics data acquisition. Numbers in parentheses indicate the number of DDA files per tissue type. B. Protein identification and iRT detection from DDA raw files using pFind. C. SiRT detection and calibration. D. CiRT detection and calibration. E. Generation of DPHL. Details of the commands are presented in File S18. DDA, data-dependent acquisition; DIA, data-independent acquisition; iRT, indexed retention time; PCT, pressure cycling technology; SCX, strong cation-exchange; SiRT, synthetic iRT; CiRT, common internal iRT; DPHL, DIA pan-human library.