Table 3.
qRT-PCR results validation by ddPCR. Condition- and allele-specific BDNF expression differences.
| Genotype | qRT-PCR | ddPCR | qRT-PCR | ddPCR | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tpre BDNF-B2M | Tpost BDNF-B2M | T BDNF post /pre | Tpre BDNF /1000B2M | Tpost BDNF /1000B2M | T BDNF post /pre | Pre MetBDNF /ValBDNF | Post MetBDNF /ValBDNF | Pre MetBDNF /ValBDNF | Post MetBDNF /ValBDNF | |
| V/V | 10.94 | 11.41 | 0.721 | 1.444 | 0.703 | 0.487 | ||||
| V/V | 11.01 | 11.66 | 0.641 | 1.792 | 1.17 | 0.653 | ||||
| V/M | 9.950 | 9.960 | 0.996 | 1.427 | 0.926 | 0.649 | 0.541 | 0.621 | 0.707 | 0.614 |
| V/V | 9.760 | 9.950 | 0.877 | 0.793 | 0.498 | 0.628 | ||||
| V/M | 10.04 | 10.86 | 0.566 | 1.524 | 0.910 | 0.598 | 0.741 | 0.662 | 0.660 | 0.968 |
| V/V | 9.250 | 10.48 | 0.427 | 2.319 | 0.745 | 0.321 | ||||
| V/V | 9.850 | 9.740 | 1.077 | 2.045 | 1.110 | 0.543 | ||||
| V/V | 8.720 | 9.300 | 0.665 | 3.460 | 1.607 | 0.464 | ||||
| Means ± SD | T BDNF post/pre, 0.736 ± 0.205 |
T BDNF post/pre, 0.543 ± 0.115 |
MetBDNF/ValBDNF, 0.641 ± 0.084 |
MetBDNF/ValBDNF, 0.737 ± 0.158 |
||||||
Pre, Analyses from the rest condition; Post, Analyses from exercise protocol; Tpre and Tpost - total (both alleles) BDNF expression, ValBDNF, MetBDNF - allele-specific BDNF expression.