Fig 4.

Naringenin treatment leads to activation of cas3 and PARP. (A, B)Images and quantification of active cas3⁺ area in B16F10 and SK-MEL-28 cells.Scale bar, 100 μm. (C-F)Images and quantification of protein expression of cas3, active cas3 and PARP, active PARP in B16F10 (C, D) and SK-MEL-28 cells (E, F) after naringenin treatment in a dose-dependent manner. Values are mean ± SD from ≥ three independent experiments. *p = 0.0340, **p = 0.0013, ***p < 0.0001; #p< 0.05, ##p = 0.01, ###p< 0.001 versus untreated cells by one-way ANOVA followed by Bonferroni post-tests.