Fig. 3.

Hypoxia regulates H3K27 acetylation at GR binding sites. (A) A Venn diagram describes overlap of GR cistromes in normoxia (green) and hypoxia (yellow). (B) UCSC browser track for GR binding at the PER1 locus, where the y axis depicts the coverage by non redundant and extended reads from MACS analysis. (C) Cistromes for H3K27ac, and p300 in HeLa cells were aligned with GR binding peaks. Two groups were observed, one showing strong overlap amongst the three signals, and one cluster showing negligible overlap. (D) H3 acetylation levels of TSC22D3, FKBP5 and MT1X were quantified by ChIP-qPCR, in response to dexamethasone treatment. Graphs illustrate fold enrichment of immunoprecipitated H3K27ac in response to treatment with dexamethasone over vehicle treated control. Mean ± S.D. from three independent experiments. *p < 0.01, NS: not significant. (E) A Venn diagram summarises the overlap of the three cistromes, where GR is shown in green, p300 in blue and H3K27ac in yellow.