| Tests of isolates |
Number of isolates in each test |
| Motility test |
Non-motile |
36 |
| Motile |
16 |
| MALDI-TOF |
Score above 2.3 |
1 identified as Bacillus anthracis
|
| Score values between 2.0-2.3 |
11 confirmed as Bacillus sp. |
| Score values between 1.7-2.0 |
22 identified as probable Bacillus sp. |
| Score values lower than 1.7 |
18 were not identified |
| Susceptibility to gamma phage and penicillin |
Susceptible to gamma phage and penicillin |
1 |
| Susceptible to gamma phage and resistant to penicillin |
10 |
| Resistant to gamma phage and penicillin |
13 |
| Resistant to gamma phage and susceptible to penicillin |
28 |
| PCR |
pXO1+/pXO2- |
02 |
| pXO1-/pXO2- |
50 |
| Amplicon sequencing |
Identity to pag (surogate of pXO1) |
Isolate 29 (100% identity) |
| Isolate 41 (99.6% identity) |
| 16sRNA |
Identified as strain belonging to B. cereus group |
Isolate 29 (100% identity and 100% query cover to B. paramycoides strain MCCC 1A04098, B. albus strain MCCC 1A02146 and B. anthracis ATCC141576 |
| Isolate 41 (100% identity and 94% query cover to eight species belonging to the B. cereus group) |
| Isolates 2, 3, 4, 6, 7, 8, 30, 36 and 37 (match to several species belonging to the B. cereus group) |
