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. Author manuscript; available in PMC: 2020 Nov 6.
Published in final edited form as: Oncogene. 2010 Jun 21;29(33):4658–4670. doi: 10.1038/onc.2010.218

Figure 1.

Figure 1

Snail2 is required for the survival of cells with mutant KRAS. (a) HCT-116 and HKe-3 cells were screened using a retrovirally delivered, doxycycline-inducible, shRNA library to identify genes required for cell survival. Depletion of cells bearing three Snail2-targeted shRNAs in shRNA-uninduced versus induced cells is plotted; error bars represent the standard deviation of triplicate measurements. (b) A Snail2 shRNA is more toxic to HCT-116 cells compared with HKe-3 and HKh-2 cells. A vector for inducible expression of Snail2 shRNA was introduced into cell lines and cell numbers were monitored at indicated days after doxycycline addition. Data were the ratio of live cell number in shRNA-induced versus uninduced cells. *P<0.05; error bars indicate s.d. (c) Snail2 knockdown causes strong inhibition effects on soft agar colony formation of HCT-116 cells. Data were the ratio of soft agar colonies in shRNA-induced versus uninduced cells. Error bars represent the s.d. of triplicates. (d) Snail2 knockdown is more toxic to SW48 KRAS G13D cells compared with SW48 wt cells. siRNAs against Snail2 were transfected into both cell lines and cell viability was measured. **P<0.01; error bars indicate s.d.