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. 2020 Nov 3;8:e10147. doi: 10.7717/peerj.10147

Figure 2. Effect of 22AA on the expression of occludin.

Figure 2

Representative western blot results are shown in A. The relative occludin expression level was calculated using the occludin/β-actin gray density ratio. The detailed values are shown in B. In the control group, the expression value was 0.9967. From 7D to 27D, the occludin expression level in the 22AA group gradually decreased to 0.1621, which was only 16.26% of that in the control group. Then, the expression level of occludin gradually increased to 0.3543 at 47D, which was approximately one-third the normal expression level (35.54%). Occludin expression was significantly different among the six groups (P < 0.05). The results showed that the expression level of occludin in the 22AA group at each time was significantly different from that in the control group. *P < 0.01.