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. Author manuscript; available in PMC: 2020 Nov 6.
Published in final edited form as: Nat Chem Biol. 2017 Apr 10;13(6):655–659. doi: 10.1038/nchembio.2348

Figure 4 |. NADP(H) binding affects both short and long range interactions in Pf NfnI.

Figure 4 |

HDX-MS shows proton exchange in backbone residues after NADPH binding (red, more change; blue, less change). Increased exchange (red) with NADP+ occurred at L-FAD toward the protein surface and along the interface between NfnI-S and NfnI-L. (b) Unbound Pf NfnI showing NADP(H) binding cavity with L-FAD (cyan) and N5 interaction region. NADP(H) binding causes a loop rearrangement (blue) as visualized by superimposition of secondary structure of the NADP(H) bound Pf NfnI. (c) NADP(H) binding cavity with bound NADPH (blue sticks) along with HDX changes (red) and structural rearrangements (blue).