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. 2020 Nov 2;13:11193–11209. doi: 10.2147/OTT.S274092

Figure 5.

Figure 5

CTHRC1 is a direct target of let-7c-5p. (A) WT and Mut sequences of the putative let-7c-5p target sequences of CTHRC1 3’-UTR. (B) Dual-luciferase activity assay showing the effect of let-7c-5p on CTHRC1 3’-UTR luciferase activity in HEK-293T cells. (C) TE-1 and KYSE150 cells were transfected with let-7c-5p mimics and mRNA levels of CTHRC1 were assessed by qRT-PCR. (D) TE-1 and KYSE150 cells were transfected with let-7c-5p inhibitor and mRNA levels of CTHRC1 were examined by qRT-PCR. (E) TE-1 and KYSE150 cells were transfected with let-7c-5p mimics or inhibitor and protein levels of CTHRC1 were analyzed by Western blot. (F) The CTHRC1/GAPDH ratio was quantified. All results are shown as the mean ± SD of three separate experiments (n=3). Statistical analysis was performed with Student’s t-test. *p < 0.05 and **p < 0.01.