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. 2020 Nov 2;13:11193–11209. doi: 10.2147/OTT.S274092

Figure 8.

Figure 8

CTHRC1 reversed let-7c-5p-mediated AKT and ERK signaling pathways. (A) mRNA levels of CTHRC1 were measured after transfection with siCTHRC1 in TE-1 cells by qRT-PCR. (B) mRNA levels of CTHRC1 were examined after transfection with siCTHRC1 in KYSE150 cells by qRT-PCR. (C) Protein levels of CTHRC1 were detected after transfection with siCTHRC1 in TE-1 and KYSE150 cells by Western blot. (D) The CTHRC1/GAPDH ratio was quantified. (E) Protein levels of AKT, p-AKT, ERK and p-ERK were measured after transfection with siCTHRC1-2 in TE-1 and KYSE150 cells by Western blot. (F) The p-AKT/AKT and p-ERK/ERK ratio were quantified. (G) Protein levels of AKT, p-AKT, ERK and p-ERK were measured after cotransfection with siCTHRC1-2 and let-7c-5p inhibitor in TE-1 and KYSE150 cells by Western blot. (H) The p-AKT/AKT and p-ERK/ERK ratio were quantified. All results are shown as the mean ± SD of three separate experiments (n=3). Statistical analysis was performed with Student’s t-test. *p < 0.05 and **p < 0.01.