TABLE 1.
MICs of antimicrobial agents against strains containing cloning fragments or plasmids
| Strain and/or plasmid | MIC (mg/liter) ofa
: |
Note | |||||
|---|---|---|---|---|---|---|---|
| ATM | ATM-AVI | ATM-AVI | CAZ | CAZ-AVI | CAZ-AVI | ||
| 035125 | 128 | 16/4 | 1/8 | 1,024 | 8/4 | 0.5/8 | ST410, YRIK insertion in PBP3, carrying blaCMY-42, from sewage |
| 035123 | 512 | 64/4 | 4/8 | >1,024 | 16/4 | 1/8 | ST410, YRIK insertion in PBP3, carrying blaCMY-42, from sewage |
| 035148 | 512 | 128/4 | 16/8 | >1,024 | 32/4 | 4/8 | ST410, YRIK insertion in PBP3, carrying blaCMY-42, from sewage |
| 005008 | >512 | 4/4 | 0.5/8 | >1,024 | 64/4 | 16/8 | ST3835, YRIN insertion in PBP3, carrying blaCMY-42 and blaNDM-1, from ascites (17) |
| 005008R1 | >512 | 64/4 | 4/8 | >1,024 | 256/4 | 32/8 | ATM-AVI-resistant mutant of 050008, SNP in inc |
| 005008R2 | >512 | 64/4 | 4/8 | >1,024 | 256/4 | 32/8 | ATM-AVI-resistant mutant of 050008, SNP in inc |
| 020066 | 512 | 8/4 | 1/8 | >1,024 | 64/4 | 16/8 | ST6823, YRIP insertion in PBP3, no blaCMY-42, carrying blaNDM-1, from urine (22) |
| BL21 | 0.015 | 0.015/4 | 0.015 | 0.015/4 | |||
| BL21:pET28a | 0.015 | 0.015/4 | 0.015 | 0.015/4 | |||
| BL21::pET28a_CMY2 | 32 | 0.015/4 | 32 | 0.015/4 | blaCMY-2 cloned on pET28a | ||
| BL21::pET28a_CMY42 | 512 | 0.03/4 | >1,024 | 0.03/4 | blaCMY-42 cloned on pET28a | ||
| 035125ΔpCMY42 | 2 | 1/4 | 4 | 1/4 | 035125 without pCMY42_035125, resulting from plasmid curation | ||
| 035125ΔpCMY42::pET28a | 2 | 1/4 | 4 | 1/4 | |||
| 035125ΔpCMY42::pET28a_CMY2 | 32 | 4/4 | 0.125/8 | 32 | 2/4 | 0.06/8 | blaCMY-2 cloned on pET28a |
| 035125ΔpCMY42::pET28a_CMY42 | 512 | 64/4 | 2/8 | >1,024 | 32/4 | 1/8 | blaCMY-42 cloned on pET28a |
| 035125ΔpCMY42::pCMY42_035125 | 128 | 16/4 | 1/8 | 1,024 | 8/4 | 0.5/8 | pCMY42_035125 introduced back into 035125ΔpCMY42 |
| 035125ΔpCMY42::pCMY42_035123 | 512 | 32/4 | 4/8 | >1,024 | 16/4 | 1/8 | pCMY42_035123 introduced into 035125ΔpCMY42 |
| 035125ΔpCMY42::pCMY42_035148 | 1,024 | 128/4 | 16/8 | >1,024 | 32/4 | 4/8 | pCMY42_035148 introduced into 035125ΔpCMY42 |
| 035125ΔpCMY42::pCMY42_005008 | 128 | 4/4 | 0.5/8 | 1,024 | 8/4 | 0.25/8 | pCMY42_005008 introduced into 035125ΔpCMY42 |
| 035125ΔpCMY42::pCMY42_005008R1 | 512 | 64/4 | 2/8 | >1,024 | 64/4 | 1/8 | pCMY42_005008R1 introduced into 035125ΔpCMY42 |
| 035125ΔpCMY42::pCMY42_005008R2 | 512 | 64/4 | 2/8 | >1,024 | 64/4 | 1/8 | pCMY42_005008R2 introduced into 035125ΔpCMY42 |
| BL21::pBC SK | 0.015 | 0.015/4 | 0.015 | 0.015/4 | |||
| BL21:: pbp3_BL21 | 0.015 | 0.015/4 | 0.015 | 0.015/4 | pbp3 of BL21 cloned on pBC SK | ||
| BL21:: pbp3_YRIN | 0.25 | 0.25/4 | 0.25 | 0.25/4 | pbp3 of 035125 cloned on pBC SK | ||
| BL21:: pbp3_YRIK | 0.25 | 0.25/4 | 0.25 | 0.25/4 | pbp3 of 005008 cloned on pBC SK | ||
| BL21:: pbp3_YRIP | 0.25 | 0.25/4 | 0.25 | 0.25/4 | pbp3 of 020066 cloned on pBC SK | ||
a
Resistance is highlighted in boldface.