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. 2020 Oct 14;57(6):1319–1332. doi: 10.3892/ijo.2020.5133

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Copyright: © Oh et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Interaction of JMJD1A with ETV1. (A) Flag-tagged JMJD proteins were co-expressed with Myc-tagged ETV1. Shown are immunoprecipitations (IP) with anti-Flag antibody followed by western blot analysis with anti-Myc antibody (top panel), as well as input levels of ETV1 (middle panel) and JMJD proteins (bottom panel). (B) Comparable degree of co-immunoprecipitation of ETV1 with wild-type JMJD1A and its H1120A/D1122G catalytic mutant (mut). (C) Endogenous coimmunoprecipitation in HCT116 cells. No, anti-ETV1 or a control antibody was utilized for immunoprecipitation. Right panel shows equal input levels of JMJD1A. (D) Interaction of purified proteins was tested by GST pull-down assays (top panel). Purity of recombinant proteins (GST, GST-ETV1 and Flag-His-JMJD1A) was assessed by Coomassie staining (bottom panel). JMJD, Jumonji C domain-containing protein.