Table I.
Sequences of primers used for gene amplification by reverse-transcription quantitative PCR.
| Genes | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') |
|---|---|---|
| β-actin | ATTGTGCACCGCAAATGCTT | ACCACAGCACGATTGTCGAT |
| HIF-1α | CCACCACAACTGCCACCACTG | TGCCACTGTATGCTGATGCCTTAG |
| SOX9 | TCAACGGCTCCAGCAAGAACAAG | CTCCGCCTCCTCCACGAAGG |
| Col2α | GGTCCTCCTGGTCCTGGCATC | CGTGCTGTCTCAAGGTACTGTCTG |
| AGG | CGTTGCAGACCAGGAGCAAT | CTCGGTCATGAAAGTGGCGG |
| MMP9 | AGAGACCACCACCACCACCAC | TGCCTGCCTCCACTCCTTCC |
| MMP13 | CTACCATCCTGCGACTCTTGCG | CCACATCAGGCACTCCACATCTTG |
| ADAMTS5 | AAGAGGAGGAGGAGGAGGAGGAG | AATGGTTGTGAGCTGCCGTATGG |
| PHD1 | AGGCTATGTCCGTCACGTTG | TGGGCTTTGCCTTCTGGAAA |
| PHD2 | ATATTGTGCCTTGCATGCGG | TGGCTCACTAGTTGCCCATC |
| PHD3 | AGGCAATGGTGGCTTGCTAT | GACCCCTCCGTGTAACTTGG |
HIF-1α, hypoxia-inducible factor-1α; AGG, aggrecan; Col2α, collagen type 2; MMP, matrix metalloproteinase; ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5; PHD, prolyl hydroxylase.