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. 2020 Oct 22;20(6):239. doi: 10.3892/etm.2020.9369

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Copyright: © Bi et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Role of siPIK3R1, miR-342-5p inhibitor and the combination of miR-342-5p inhibitor with siPIK3R1 on cell proliferation and migration. (A) Transfection efficiency of siPIK3R1 was determined using RT-qPCR. (B) The mRNA expression levels of miR-342-5p were detected using RT-qPCR following transfection. (C) A Cell Counting Kit-8 assay was used to determine cell proliferation. (D) Quantification of cell migration rates in 5 experimental groups. (E) Representative cell migration in 5 experimental groups detected using a wound healing assay. Magnification, x200. ^{^^}P<0.01 vs. siNC; ^*P<0.05, ^**P<0.01 vs. NC; ^##P<0.01 vs. NC + siPIK3R1. miR, microRNA; siPIK3R1, si, small interfering; PIK3R1, phosphatidylinositol 3-kinase regulatory subunit α; NC, miR-342-5p inhibitor negative control; RT-qPCR, reverse transcription-quantitative PCR.