Table I.
Sequences of primers used for reverse transcription-quantitative PCR.
| Gene | Sequence (5′→3′) |
|---|---|
| Col1a1 | F: GCTCCTCTTAGGGGCCACT |
| R: ATTGGGGACCCTTAGGCCAT | |
| Col1a2 | F: TCGTGCCTAGCAACATGCC |
| R: TTTGTCAGAATACTGAGCAGCAA | |
| CTGF | F: GGCCTCTTCTGCGATTTCG |
| R: GCAGCTTGACCCTTCTCGG | |
| PAI-1 | F: GGCCTCTTCTGCGATTTCG |
| R: GCAGCTTGACCCTTCTCGG | |
| α-SMA | F: AGAAACGGGACAAACTTCGTC |
| R: GTCTTGCACTGTATAGCCGAG | |
| TGF-β1 | F: CCACCTGCAAGACCATCGAC |
| R: CTGGCGAGCCTTAGTTTGGAC | |
| IL-1β | F: GAAATGCCACCTTTTGACAGTG |
| R: TGGATGCTCTCATCAGGACAG | |
| IL-4 | F: CCCCAGCTAGTTGTCATCCTG |
| R: CAAGTGATTTTTGTCGCATCCG | |
| IL-6 | F: CTGCAAGAGACTTCCATCCAG |
| R: AGTGGTATAGACAGGTCTGTTGG | |
| TNF-α | F: CAGGCGGTGCCTATGTCTC |
| R: CGATCACCCCGAAGTTCAGTAG | |
| MCP-1 | F: TAAAAACCTGGATCGGAACCAAA |
| R: GCATTAGCTTCAGATTTACGGGT | |
| GAPDH | F: AGGTCGGTGTGAACGGATTTG |
| R: GGGGTCGTTGATGGCAACA |
Col1a1, collagen type I alpha 1 chain; Col1a2, collagen type I alpha 2 chain; CTGF, connective tissue growth factor; PAI-1, plasminogen activator inhibitor-1; α-SMA, α-smooth muscle actin; MCP-1, monocyte chemoattractant protein-1; F, forward; R, reverse.