Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 28;22(6):4810–4820. doi: 10.3892/mmr.2020.11541

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Xiao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 2. — Exosomal circ_400068 enhances the proliferation and suppresses the apoptosis of kidney cells, and the effects are reversed by sh-circ_400068. (A) Expression of circ_400068 was elevated in HK-2 cells treated with RCC-Exo. (B) Transfection efficiency of sh-circ_400068 in HK-2 cells was assessed using reverse transcription quantitative PCR compared with the sh-NC. (C) Proliferative activity in HK-2 cells were enhanced following the treatment with RCC-Exo, which was abrogated by sh-circ_400068. (D) The RNA levels of Ki-67 were increased in cells treated with RCC-exo and reduced by sh-circ_400068. (E) The protein levels of Ki-67 were enhanced in cells treated with RCC-exo, which was reversed by sh-circ_400068. (F) Flow cytometry results demonstrated that the (G) cell apoptotic rate was significantly reduced in RCC-Exo-treated HK-2 cells, and the effects were rescued by the treatment with sh-circ_400068. (H) Western blotting results indicated that the (I) expression of cleaved caspase-7 was decreased in HK-2 cells treated with RCC-Exo, which was abrogated by sh-circ_400068. *P<0.05 vs. NC. NC, negative control; sh-, short hairpin RNA; RCC-Exo, exosomes isolated from the culture media of RCC cells; circ, circular RNA; RCC, RCC, renal cell carcinoma; OD, optical density.