Figure 4.

SOCS1 is a putative target of miR-210-5p. (A) Novel binding sites of SOCS1 on the miR-210-5p transcript. (B) Transfection with miR-210-5p mimics resulted in significant decrease in the luciferase activity of WT-SOCS1, while no decrease was detected in MUT-SOCS1 group. (C) SOCS1 expression was evaluated in HK-2 cells transfected with miR-210-5p mimics and inhibitors using reverse transcription-quantitative PCR compared with miR-NC. (D) Protein expression levels of SOCS1 and its downstream molecules in transfected HK-2 cells were examined using western blotting compared with miR-NC. *P<0.05 vs. miR-NC. RCC, renal cell carcinoma; miR, microRNA; NC, negative control; MUT, mutant; WT, wild-type; SOCS1, suppressor of cytokine signaling 1.