Figure 1.

Mtp regulates HUVEC viability and senescence. (A) Following treatment with Mtp for 24 h, HUVEC viability was assessed by performing an MTT assay. (B) Following pretreatment with Mtp for 24 h and incubation with H₂O₂ for 12 h, HUVEC viability was assessed by performing an MTT assay. (C) Following pretreatment with 100 µM Mtp for 24 h and incubation with 100 µM H₂O₂ for 12 h, β-Gal activity was measured using a β-Gal assay kit. (D) The protein expression levels of Hmga1, γ-H2AX and H2AX were measured via western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. control; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. H₂O₂. Mtp, monotropein; HUVEC, human umbilical vein endothelial cell; β-Gal, β-galactosidase; Hmga1, high mobility group AT-hook 1; H2AX, H2A.X variant histone.