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. 2020 Sep 29;22(6):4828–4836. doi: 10.3892/mmr.2020.11548

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — HUVEC oxidative stress and apoptosis are suppressed by Mtp. (A) MDA levels, and (B) SOD and (C) GSH-Px activities were determined using corresponding assay kits. (D) The TUNEL assay was performed to identify apoptotic cells (magnification, ×200). Blue-stained cells represent normal HUVECs and brown-stained cells indicate apoptotic cells. (E) The protein expression levels of Bcl-2, Bax, cleaved-caspase 3 and caspase 3 were measured via western blotting. ***P<0.001 vs. control; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. H₂O₂. HUVEC, human umbilical vein endothelial cell; Mtp, monotropein; MDA, malondialdehyde; SOD, superoxide dismutase; GSH-Px, glutathione peroxidase.