Figure 6.

Effect of apigenin on the apoptotic pathway in melanoma cells. (A) A375P and (B) A375SM melanoma cells were treated with apigenin (0, 50, 100 µM) for 24 h and cells were harvested to measure protein levels of Bax, Bcl-2, PARP, p53 and caspase-9 by western blotting. Blots were also probed with β-actin antibody to confirm equal sample loading. Data are presented as the mean ± standard deviation from three independent experiments. Significance was determined using ANOVA followed by a Dunnett's post hoc test. *P<0.05 vs. untreated control group. PARP, poly ADP-ribose polymerase.