Figure 9.

Effect of apigenin on p-ERK expression in A375SM-induced melanoma tumors. Nude mice were administered with apigenin for 3 weeks and the expression of p-ERK was assessed by immunohistochemistry. Paraffin-embedded tumor tissues were cut into 5-µm-thick sections and tissues were observed under a microscope and photographed (magnification, ×200; scale bar, 10 µm). Each bar represents the p-ERK-positive cells (relative to control). Data are presented as the mean ± standard deviation from three independent experiments. Significance was determined using ANOVA followed by a Dunnett's post hoc test. *P<0.05 vs. untreated control group. ERK, extracellular signal-regulated protein kinase; p-, phosphorylated.