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Primer sequences used for quantitative PCR.
| Gene | Forward sequence (5′→3′) | Reverse sequence (5′→3′) |
|---|---|---|
| NLRP3 | CTCTGCATGCCGTATCTGGT | GTCCTGAGCCATGGAAGCAA |
| Caspase-1 | GACCGAGTGGTTCCCTCAAG | GACGTGTACGAGTGGGTGTT |
| ASC | GGACAGTACCAGGCAGTTCG | GTCACCAAGTAGGGCTGTGT |
| IL-1β | GACTTCACCATGGAACCCGT | GGAGACTGCCCATTCTCGAC |
| GAPDH | GGCAAATTCAACGGCACAGT | AGATGGTGATGGGCTTCCC |
ASC, apoptosis-associated speck-like protein; NLRP3, NLR family pyrin domain-containing 3.
