Skip to main content
. Author manuscript; available in PMC: 2021 Oct 21.
Published in final edited form as: Org Biomol Chem. 2020 Oct 21;18(40):8094–8102. doi: 10.1039/d0ob01736a

Table 1.

Sulfotransferase activities for 6-OST wild-type and mutant enzymes

Name Mutation sites Sulfotransferase activity (pmoles/μg/h)*
6-OST WT Not applicable 72.3
6-OST Mt-1 6-OST R112E 40.2
6-OST Mt-2 6-OST R112E/R206E 27.8
6-OST Mt-3 6OST R112E/R329E 73.5
6-OST Mt-4 6-OST R112E/R206E/R329E
 12.6
*

Sulfotransferase activity was determined by measuring the amount of 35S-labeled sulfate transferred from [35S]PAPS to completely desulfated/N-sulfated (CDSNS) heparin, a structurally heterogeneous polysaccharide substrate.