| Antibodies |
| Rabbit monoclonal anti-STING |
Cell Signaling Technology |
Cat# 13647 |
| Mouse monoclonal anti-β-actin |
Santa Cruz |
sc-47778 |
| Bacterial Strains |
| Brucella melitensis 16M |
UW-Madison archive |
N/A |
| Brucella abortus 2308 |
UW-Madison archive |
N/A |
|
Brucella neotomae Stoenner and Lackman 1957 |
ATC 23459 |
N/A |
| Brucella suis 1330 |
UW-Madison archive |
N/A |
| Escherichia coli DH5a |
UW-Madison archive |
N/A |
| Chemicals |
| 4μ8c |
EMD Millipore |
Cat#412512-25MG |
| ODN 1585 |
Invivogen |
Tlrl-1585 |
| Pam3CSK4 |
Invivogen |
Tlrl-pms |
| Critical Commercial Assays |
| RNAzol RT |
Molecular Research Center, Inc. |
RN 190 |
| PowerUp SYBR Green Master Mix |
AppliedBiosystems |
Cat# A25742 |
| B-PER Bacterial Protein Extraction Reagent |
ThermoFisher Scientific |
Cat# 78248 |
| M-PER Mammalian Protein Extraction Reagent |
ThermoFisher Scientific |
Cat# 78501 |
| Halt Protease and Phosphatase Inhibitor |
ThermoFisher Scientific |
Cat# 78440 |
| Dual-Glo Luciferase assay system |
Promega |
Cat# E2490 |
| Cell Line Nucleofector kit V |
Lonza |
VVCA-1003 |
| Lipofectamine RNAiMAX Transfection Reagent |
ThermoFisher Scientific |
Cat# 13778030 |
| Opti-MEM |
ThermoFisher Scientific |
Cat# 31985070 |
| LEGEND MAX Mouse IFN-β ELISA Kit with Pre-coated Plates |
BioLegend |
Cat# 439407 |
| qScript microRNA cDNA Synthesis Kit |
Quanta Biosciences |
Cat# 95107–025 |
| SuperScript IV VILO Master Mix |
ThermoFisher Scientific |
Cat# 11756050 |
| LPS Extraction Kit |
iNtRON Biotechnology |
Cat# 17141 |
| Experimental Models: Cell Lines |
| iMACs (immortalized macrophages) |
John-Demian Sauer |
N/A |
| LADMAC (for CSF-1) |
ATCC |
CRL-2420 |
| Experimental Models: Organisms/Strains |
|
Mus musculus C57BL/6 |
Federal University of Minas Gerais; IUSM South Bend |
N/A |
|
Mus musculus C57BL/6 STING-/-
|
Ishikawa and Barber, 2008 |
N/A |
| Mus musculus mirn23a-/-(on C57BL/6 background) |
Richard Dahl; IUSM South Bend |
N/A |
| Mus musculus MyD88-/-(on C57BL/6 background) |
Bruce Klein |
N/A |
| Oligonucleotides and plasmids |
| mirVana miRNA inhibitor hsa-miR-24-3p |
ThermoFisher Scientific |
Cat# 4464084 |
| Anti-miR miRNA Inhibitor Negative Control #1 |
ThermoFisher Scientific |
Cat# AM17010 |
| miRNA Neg Control |
SIGMA |
MISSION miRNA Control |
| miRNA hsa-mir-24 |
SIGMA |
MISSION miRNA Mimic |
| miRNA inhibitor mmu-mir-24-3p |
SIGMA |
MISSION miRNA Inhibitor |
| VirB2 knock-out vector, pAV2.2 |
Renee M. Tsolis |
Den Hartigh et al., 2004 [22] |
| pBBR1-MCS4 |
Lab Archive |
N/A |
| pBBR1-VirB2 |
This work |
N/A |
| pSTING-254 murine STING promoter luciferase plasmid |
Hua-Guo Xu |
Xu et al., 2017 [67] |
| Software and Algorithms |
|
|
| GraphPad Prism 7 |
Graphpad Software |
N/A |
