Skip to main content
. 2020 Oct 27;16(10):e1009157. doi: 10.1371/journal.pgen.1009157

Fig 5. OsTIF1 transfers from the endoplasmic reticulum (ER) into the nucleus after IAA releases its interaction with OsAsp1.

Fig 5

A, OsAsp1 co-localised with OsTIF1 in the ER of rice mesophyll protoplast. The ER marker was ER-rk CD3-959, purchased from the Arabidopsis Stock Center (http://www.arabidopsis.org/). Scale bars, 5 μm. B, OsTIF1, but not OsAsp1, was transferred from the ER into the nucleus in rice mesophyll protoplasts in response to IAA treatment (50 μM). The nuclear marker was AtBZR2(1–158 aa)-mCherry. Scale bars, 5 μm. C, Interaction between OsAsp1 and OsTIF1 detected by yeast two-hybrid assay.–LH indicates SD medium without LEU and TRP, and–LTH indicates SD medium without LEU, TRP, and HIS. D, Interaction between OsAsp1 and OsTIF1 detected by a Co-IP assay. PUBI:OsTIF1-HA was co-transformed with PUBI:OsAsp1-GFP or PUBI:GFP into rice mesophyll protoplast, and then IAA (50 μM) was added, along with OsTIF1-HA as bait to pull down OsAsp1-GFP. Anti-GFP (a-GFP) was used to detect OsAsp1-GFP or green fluorescent protein (GFP), and anti- hemagglutinin (a-HA) was used to detect OsTIF1-HA.