Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
nSybGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_51635 | |
| Genetic reagent (D. melanogaster) |
UAS GCaMP6m | Bloomington Drosophila Stock Center | RRID:BDSC_42750 | |
| Genetic reagent (D. melanogaster) |
UAS GCaMP6m | Bloomington Drosophila Stock Center | RRID:BDSC_42748 | |
| Genetic reagent (D. melanogaster) |
UAS Arclight | Bloomington Drosophila Stock Center | RRID:BDSC_51056 | |
| Genetic reagent (D. melanogaster) |
UAS mCD8GFP | Bloomington Drosophila Stock Center | RRID:BDSC_5130 | |
| Genetic reagent (D. melanogaster) | UAS Dicer | Bloomington Drosophila Stock Center | RRID:BDSC_24648 | |
| Genetic reagent (D. melanogaster) | UAS Chrimson | Bloomington Drosophila Stock Center | RRID:BDSC_55137 | |
| Genetic reagent (D. melanogaster) | UAS syt.eGFP | Bloomington Drosophila Stock Center | RRID:BDSC_6926 | |
| Genetic reagent(D. melanogaster) | UAS RFP | Bloomington Drosophila Stock Center | RRID:BDSC_32218 | |
| Genetic reagent (D. melanogaster) | MB058BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68278 | |
| Genetic reagent (D. melanogaster) | MB296BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_63308 | |
| Genetic reagent (D. melanogaster) | MB304BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68367 | |
| Genetic reagent (D. melanogaster) | MB320CGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68253 | |
| Genetic reagent (D. melanogaster) | MB630BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68334 | |
| Genetic reagent (D. melanogaster) | MB438BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68326 | |
| Genetic reagent (D. melanogaster) | MB504BGAL4 | Bloomington Drosophila Stock Center | RRID:BDSC_68329 | |
| Genetic reagent (D. melanogaster) | UAS mAChR RNAi (dsmAChR) | Vienna Drosophila Resource Center RRID:SCR_013805 |
VDRC_101407 | |
| Genetic reagent (D. melanogaster) | UAS itprRNAi (dsitpr) | National Institute of Genetics | NIG_1063 R-2 | |
| Genetic reagent (D. melanogaster) | UAS Itpr+ | Venkatesh et al., 2001 | RRID:BBSC_30742 | |
| Genetic reagent (D. melanogaster) | THGAL4 | Friggi-Grelin et al., 2003 (DOI:10.1002/neu.10185) | Gift from Serge Birman (CNRS, ESPCI Paris Tech, France) | |
| Genetic reagent (D. melanogaster) | THD’ GAL4 | Liu et al., 2012 (DOI:10.1016/j.cub.2012.09.008) | Gift from Mark N Wu (Johns Hopkins University, Baltimore) | |
| Genetic reagent (D. melanogaster) | THD1 GAL4 | Liu et al., 2012 (DOI:10.1016/j.cub.2012.09.008) | Gift from Mark N Wu (Johns Hopkins University, Baltimore) | |
| Genetic reagent (D. melanogaster) | THC’ GAL4 | Liu et al., 2012 (DOI:10.1016/j.cub.2012.09.008) | Gift from Mark N Wu (Johns Hopkins University, Baltimore) | |
| Genetic reagent (D. melanogaster) | UAS GRABDA | Sun et al., 2018 (DOI:10.1016/j.cell.2018.06.042) | Gift from Yulong Li (Peking University School of Life Sciences, Beijing, China) | |
| Genetic reagent (D. melanogaster) | UAS Shibirets | Kitamoto, 2001 (DOI:10.1002/neu.1018) | Gift from Toshihiro Kitamoto (University of Iowa, Carver College of Medicine, Iowa) | |
| Genetic reagent (D. melanogaster) | vGlutVGN6341GAL4 | Syed et al., 2016 (DOI:10.7554/eLife.11572) | Gift from K. Vijayraghavan (NCBS, India) | |
| Genetic reagent (D. melanogaster) | UAS mitoGCaMP | Lutas et al., 2012 (DOI:10.1534/g3.111.001586) | Gift from Fumiko Kawasaki (Pennsylvania State University, Pennsylvania) | |
| Genetic reagent (D. melanogaster) | UAS TubGAL80ts | Pathak et al., 2015 (DOI:10.1523/JNEUROSCI.1680–15.2015) | Generated by Albert Chiang, NCBS, Bangalore, India | |
| Genetic reagent (D. melanogaster) | UAS ItprDN | This paper | Transgenic Drosophila with a Dominant negative Drosophila IP3R cDNA | |
| Genetic reagent (D. melanogaster) | itpr gene mutant (itprug3) | Joshi et al., 2004 (DOI:10.1534/genetics.166.1.225) | RRID:BDSC_30738 | |
| Genetic reagent (D. melanogaster) | itpr gene mutant (itprka1091) | Joshi et al., 2004 (DOI:10.1534/genetics.166.1.225) | RRID:BDSC_30739 | |
| Antibody | Rabbit anti GFP (polyclonal) | Life Technologies, Thermo Fisher | RRID:AB_221570,Cat # A-6455 | IHC: 1:10,000 |
| Antibody | Mouse anti-bruchpilot (monoclonal) | Wagh et al., 2006 (DOI:10.1016/j.neuron.2006.02.008) | Gift from Eric Buchner, University of Wuerzburg, Germany (dilution 1:150) | |
| Antibody | Anti-mouse Alexa Fluor 568 (polyclonal) | Life Technologies, ThermoFisher Scientific | Cat# A-11004, RRID:AB_2534072 | IHC: 1:400 |
| Antibody | Anti-rabbit Alexa Fluor 488 (polyclonal) | Life Technologies, ThermoFisher Scientific | Cat# A-11008, RRID:AB_143165 | IHC: 1:400 |
| Antibody | Rabbit anti-IP3R (polyclonal) | Agrawal et al., 2009 (DOI:10.1371/journal.pone.0006652) | IB-9075 | WB: 1:300; Gift from Ilya Bezprozvanny (UT South Western, USA). |
| Antibody | Mouse anti-spectrin (monoclonal) | Developmental Studies Hybridoma Bank | Cat# 3A9 (323 or M10-2), RRID:AB_528473 | WB: 1:50 |
| Antibody | Anti-mouse HRP (polyclonal) | Cell Signaling Technology | Cat# 7076, RRID:AB_330924 | WB: 1:3000 |
| Antibody | Anti-rabbit HRP (polyclonal) | ThermoFisher Scientific | Cat#32260, RRID:AB_1965959 | WB: 1:5000 |
| Chemical compound, drug | Low melt Agar | Invitrogen | Cat# 16520–050 | |
| Chemical compound, drug | Carbachol | Sigma Aldrich | Cat# C4382 | |
| Chemical compound, drug | All trans Retinal | Sigma Aldrich | Cat# R2500 | |
| Chemical compound, drug | EcoR1 | New England Biolabs | Cat# R0101S | Restriction enzyme |
| Chemical compound, drug | Xho1 | New England Biolabs | Cat# R0146S | Restriction enzyme |
| Chemical compound, drug | AatII | New England Biolabs | Cat# R0117S | Restriction enzyme |
| Chemical compound, drug | Eag1 | New England Biolabs | Cat# R0505S | Restriction enzyme |
| Strain, strain background (Escherichia coli) | Sure Competent cells | Stratagene, Agilent Technologies | Cat# 200238 | Maintained in the lab |
| Commercial assay or kit | Quick Ligation Kit | New England Biolabs | Cat# M2200S | |
| Commercial assay or kit | WesternBright ECL kit | Advansta | Cat# K-12045-D20 | |
| Commercial assay or kit | QuikChange II XL Site-Directed Mutagenesis Kit | Agilent | Cat# 200522 | |
| Software, algorithm | Origin 8 | Origin lab | RRID:SCR_014212 | |
| Software, algorithm | Fiji/ImageJ | National Institutes of Health | RRID:SCR_002285 |