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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Thromb Haemost. 2020 Apr 9;18(6):1370–1380. doi: 10.1111/jth.14790

Fig. 2.

Fig. 2.

gC enhances HSV1-initiated FX activation when viral TF is present. (A and B) Purified FX (100 nM) and FVIIa (10 nM) were combined with HSV1/TF+/gC+ (●), HSV1/TF+/gC- (○), HSV1/TF-/gC+ (◼) and HSV1/TF-/gC- (◻) viruses in the presence of calcium (5 mM) and FXa generation was followed using the chromogenic substrate S-2765. (C and D) FX activation by HSV1 was followed as in (A and B) and the effect of adding purified gCΔ457t to the panel of HSV1 (1 × 105 vp/μL) was monitored. (E) FX activation with 1 × 105 vp/μL HSV1/TF+/gC+, HSV1/TF+/gC-, HSV1/TF-/gC+ and HSV1/TF-/gC- with (first bar) or without FX, FVIIa or calcium (following bars in order). (A - D: n = 4 ± SEM, * P < 0.05, ** P < 0.01; E: Virus and no FVIIa: n = 4 ± SEM, no FX or no Ca2+: n = 2 ± SD; error bars may be smaller than the size of symbols).