Figure 1.
lncRNA ZNF649-AS1 Is Upregulated in Trastuzumab-Resistant Breast Cancer Cells
(A and B) Gene expression variations between SKBR-3-TR and SKBR-3 cells were screened by RNA sequencing and presented as a scatterplot (A) and volcano plot (B). NC, negative control, BC, breast cancer. (C) Heatmap was used to show the 639 lncRNAs with significant differential expression (p < 0.05 and log2[fold change] > 2). (D) The top 20 most upregulated and 20 downregulated lncRNAs and shown in a heatmap. (E) The expression levels of AC010789.1, AL590483.1, Linc02474, FOXD3-AS1, and ZNF649-AS1 were detected via quantitative real-time PCR in 30 paired breast cancer tissues and adjacent non-tumor tissues. (F) Quantitative real-time PCR was used for the detection of ZNF649-AS1 in trastuzumab-resistant and parental cells. ∗p < 0.05, ∗∗p < 0.01.