Figure 4.

Silencing lncRNA ZNF649-AS1 Reverses Trastuzumab Resistance of Breast Cancer Cells

(A) ZNF649-AS1 was silenced by two specific small interfering oligonucleotides in SKBR-3-TR and BT474-TR cells. (B) RTCA of cell viability in breast cancer cells silenced with ZNF649-AS1. (C) TUNEL assay showed that silence of ZNF649-AS1 promoted cell apoptosis compared to control cells. (D) Quantitative real-time PCR verified the upregulation of ZNF649-AS1 after transfection of ZNF649-AS1-loaded plasmids in SKBR-3 and BT474 cells. (E and F) Overexpression of ZNF649-AS1 increased cell viability (E) and suppressed apoptosis (F) in cells treated with trastuzumab. ∗∗p < 0.01, ∗∗∗p < 0.001, compared to control groups.