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. 2020 Jul 21;28(11):2488–2502. doi: 10.1016/j.ymthe.2020.07.019

Figure 5.

Figure 5

Trastuzumab Treatment Induced Autophagy via lncRNA ZNF649-AS1

(A) GO pathway analysis showed that ZNF649-AS1 is associated with autophagy regulation. (B) Western blot showed that trastuzumab-resistant cells induced increased the proportion of LC3II/LC3I and decreased p62 expression. (C and D) Trastuzumab-resistant cells expressed increased LC3 puncta (C) and autophagosomes formation (D). (E) CQ treatment suppressed LC3II/LC3I proportion and increased p62 expression. (F) CCK-8 assay revealed that CQ treatment suppressed cell viability. (G) Western blot showed that knockdown of ZNF649-AS1 suppressed autophagy activity. (H and I) GFP staining suggested that ZNF649-AS1 positively regulated LC3 puncta (H) and autophagosomes formation (I). ∗∗p < 0.01, ∗∗∗p < 0.001.