Table 1.
Composition and nutrient levels of the experimental diets (%, as-fed basis).
| Item | Starter phase (days 1–21) |
Grower phase (days 22–42) |
||||||
|---|---|---|---|---|---|---|---|---|
| NCaP1 | LCaP1 | NCaP + 25-OH-D31 | LCaP + 25-OH-D31 | NCaP | LCaP | NCaP + 25-OH-D3 | LCaP + 25-OH-D3 | |
| Ingredient | ||||||||
| Corn | 58.17 | 58.17 | 58.17 | 58.17 | 64.26 | 64.26 | 64.26 | 64.26 |
| Soybean meal | 30.44 | 30.44 | 30.44 | 30.44 | 24.05 | 24.05 | 24.05 | 24.05 |
| Corn starch | - | 2.28 | - | 2.28 | - | 1.99 | - | 1.99 |
| Corn gluten meal | 2.00 | 2.00 | 2.00 | 2.00 | 2.50 | 2.50 | 2.50 | 2.50 |
| Fish meal | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Soybean oil | 3.38 | 2.35 | 3.38 | 2.35 | 3.60 | 2.70 | 3.60 | 2.70 |
| Dicalcium phosphate | 1.50 | 0.45 | 1.50 | 0.45 | 1.04 | 0.20 | 1.04 | 0.20 |
| Limestone | 1.30 | 1.10 | 1.30 | 1.10 | 1.35 | 1.10 | 1.35 | 1.10 |
| Salt | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 | 0.30 |
| L-Lys HCl, 98% | 0.01 | 0.01 | 0.01 | 0.01 | 0.08 | 0.08 | 0.08 | 0.08 |
| DL-Met, 98% | 0.14 | 0.14 | 0.14 | 0.14 | 0.04 | 0.04 | 0.04 | 0.04 |
| L-Thr, 98% | 0.01 | 0.01 | 0.01 | 0.01 | 0.03 | 0.03 | 0.03 | 0.03 |
| Chromium oxide | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 |
| Premix2 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| 25-OH-D3, μg/kg | - | - | 69 | 69 | - | - | 69 | 69 |
| Calculated levels | ||||||||
| ME, Mcal/kg | 3.05 | 3.05 | 3.05 | 3.05 | 3.15 | 3.15 | 3.15 | 3.15 |
| CP | 21.00 | 21.00 | 21.00 | 21.00 | 19.00 | 19.00 | 19.00 | 19.00 |
| Lys | 1.10 | 1.10 | 1.10 | 1.10 | 1.00 | 1.00 | 1.00 | 1.00 |
| Ca | 1.00 | 0.70 | 1.00 | 0.70 | 0.90 | 0.62 | 0.90 | 0.62 |
| Total P | 0.70 | 0.50 | 0.70 | 0.50 | 0.59 | 0.43 | 0.59 | 0.43 |
| NPP | 0.45 | 0.25 | 0.45 | 0.25 | 0.35 | 0.19 | 0.35 | 0.19 |
| Analyzed levels | ||||||||
| Ca | 0.97 | 0.68 | 0.98 | 0.69 | 0.88 | 0.60 | 0.87 | 0.61 |
| Total P | 0.68 | 0.48 | 0.68 | 0.49 | 0.57 | 0.43 | 0.56 | 0.42 |
| VD3, μg/kg | 71.9 | 72.0 | 71.8 | 71.8 | 71.7 | 71.8 | 71.7 | 71.7 |
| 25-OH-D3, μg/kg | ND3 | ND | 70.5 | 70.1 | ND | ND | 69.8 | 69.9 |
NCaP, normal calcium and phosphorus diet; LCaP, low calcium and phosphorus diet; NCaP + 25-OH-D3, normal calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol; LCaP + 25-OH-D3, low calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol.
The premix provided the following per kilogram of diet: zinc, 60 mg; iron, 100 mg; manganese, 80 mg; copper, 10 mg; iodine, 0.35 mg; selenium, 0.3 mg; vitamin A, 10,000 IU; vitamin D3, 2,760 IU; vitamin E, 30 IU; vitamin K3, 2 mg; vitamin B12, 1.2 mg; riboflavin, 6 mg; nicotinic acid, 40 mg; pantothenic acid, 12 mg; pyridoxine, 3 mg; biotin, 0.2 mg; and choline chloride, 800 mg.
Not detected.