. 2020 Aug 5;99(11):5663–5672. doi: 10.1016/j.psj.2020.07.008

Table 3.

Effects of 25-hydroxycholecalciferol supplementation on antioxidant enzyme levels in serum of broilers fed normal or low calcium and phosphorus diet.¹

Item	T-AOC (U/mL)	CAT (U/mL)	GSH-Px (U/mL)	SOD (U/mL)	MDA (nmol/mL)
Day 21
Main effect
CaP level
Normal	45.09	58.90	892	35.88	4.51
Low	34.14	54.24	814	33.47	4.12
25-OH-D₃
−	35.69	54.62	768	33.68	5.17
+	43.54	58.51	937	35.67	3.47
Interaction effect
NCaP	45.34^a	60.39^a	869^a
LCaP	26.03^b	48.85^b	667^b
NCaP + 25-OH-D₃	44.84^a	57.40^a	914^a
LCaP + 25-OH-D₃	42.23^a	59.62^a	961^a
SEM	1.23	1.88	40.60	4.18	0.37
P-value
CaP level	<0.001	0.025	0.074	0.567	0.227
25-OH-D₃	<0.001	0.055	<0.001	0.643	<0.001
CaP × 25-OH-D₃	<0.001	0.002	0.007	0.232	0.920
Day 42
Main effect
CaP level
Normal	9.20	58.44	871	29.12	5.37
Low	8.78	49.91	858	28.42	5.01
25-OH-D₃
−	8.87	52.60	868	28.22	5.54
+	9.11	55.75	861	29.33	4.83
Interaction effect
NCaP	9.28^a	60.84^a
LCaP	8.47^b	44.37^b
NCaP + 25-OH-D₃	9.13^a,b	56.05^a,b
LCaP + 25-OH-D₃	9.08^a,b	55.46^a,b
SEM	0.21	3.36	16.50	0.64	0.46
P-value
CaP level	0.025	0.022	0.462	0.354	0.457
25-OH-D₃	0.195	0.363	0.658	0.153	0.149
CaP × 25-OH-D₃	0.041	0.031	0.510	0.089	0.407

^a,bWithin comparisons, means in the same column with different superscripts differ significantly (P < 0.05).

Data were the means and SEM, n = 6 broilers per treatment for data on interaction effect, and n = 12 broilers per treatment for data on main effect.

NCaP, normal calcium and phosphorus diet; LCaP, low calcium and phosphorus diet; NCaP +25-OH-D₃, normal calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol; LCaP +25-OH-D₃, low calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol; T-AOC, total antioxidant capacity; CAT, catalase; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase; MDA, malondialdehyde.