Table 5.
Effects of 25-hydroxycholecalciferol supplementation on tibia mineralization of broilers fed normal or low calcium and phosphorus diet.1
| Item | Weight (g) | Length (cm) | Bone density (g/cm3) | Ash (%) | Ca (%) | P (%) |
|---|---|---|---|---|---|---|
| Main effect | ||||||
| CaP level | ||||||
| Normal | 4.38 | 10.26 | 1.38 | 36.84 | 17.14 | 8.22 |
| Low | 4.13 | 10.09 | 1.27 | 32.89 | 16.26 | 6.98 |
| 25-OH-D3 | ||||||
| − | 4.16 | 10.14 | 1.23 | 34.16 | 16.44 | 7.28 |
| + | 4.36 | 10.21 | 1.42 | 36.27 | 16.96 | 7.92 |
| Interaction effect | ||||||
| NCaP | 17.27a | |||||
| LCaP | 15.60b | |||||
| NCaP + 25-OH-D3 | 17.00a | |||||
| LCaP + 25-OH-D3 | 16.92a | |||||
| SEM | 0.25 | 0.16 | 0.07 | 1.60 | 0.30 | 0.46 |
| P-value | ||||||
| CaP level | 0.334 | 0.291 | 0.106 | 0.023 | 0.008 | 0.013 |
| 25-OH-D3 | 0.431 | 0.647 | 0.010 | 0.094 | 0.092 | 0.172 |
| CaP × 25-OH-D3 | 0.384 | 0.150 | 0.103 | 0.848 | 0.014 | 0.242 |
a,bWithin comparisons, means in the same column with different superscripts differ significantly (P < 0.05). Ash, Ca and P contents are calculated by dividing the ash, Ca, and P weights by fat-free dry weight of tibias.
Data were the means and SEM, n = 6 broilers per treatment for data on interaction effect, and n = 12 broilers per treatment for data on main effect.
NCaP, normal calcium and phosphorus diet; LCaP, low calcium and phosphorus diet; NCaP +25-OH-D3, normal calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol; LCaP +25-OH-D3, low calcium and phosphorus diet supplemented with 69 μg/kg 25-hydroxycholecalciferol; Ca, calcium; P, phosphorus.