Table 7.

Effects of 25-hydroxycholecalciferol supplementation on tibia biomechanical parameters of broilers fed normal or low calcium and phosphorus diet.

Item	Breaking strength (N)	Failure deflection (mm)	Stiffness (N/mm)	Absorbed energy (J)
Main effect
CaP level
Normal	305	2.04	188	0.36
Low	179	2.29	108	0.25
25-OH-D3
−	215	2.16	134	0.27
+	269	2.16	162	0.34
SEM	23.76	0.15	17.69	0.04
P-value
CaP level	<0.001	0.125	<0.001	0.017
25-OH-D3	0.034	0.988	0.124	0.097
CaP × 25-OH-D3	0.200	0.184	0.144	0.684

Data were the means and SEM, n = 6 broilers per treatment for data on interaction effect, and n = 12 broilers per treatment for data on main effect.

Abbreviation: 25-OH-D₃, 25-hydroxycholecalciferol.