Table 2.
Primer sequence of target and reference genes.
| Gene name1 | Primers sequence (5′-3′) | Accession number | |
|---|---|---|---|
| Antioxidant-related genes | |||
| NRF2 | Forward | GAGCCCATGGCCTTTCCTAT | NM_001007858.1 |
| Reverse | CACAGAGGCCCTGACTCAAA | ||
| HO-1 | Forward | AAACTTCGCAGCCACACAAC | NM_205344.1 |
| Reverse | GACCAGCTTGAACTCGTGGA | ||
| GPX | Forward | GACCAACCCGCAGTACATCA | NM_001277853.1 |
| Reverse | GAGGTGCGGGCTTTCCTTTA | ||
| SOD1 | Forward | CCGGCTTGTCTGATGGAGAT | NM_205064.1 |
| Reverse | TGCATCTTTTGGTCCACCGT | ||
| CAT | Forward | GCGCCCCGAACTATTATCCA | NM_001031215.2 |
| Reverse | ATACGTGCGCCATAGTC | ||
| Lipid metabolism genes | |||
| AMPKα | Forward | GGGACCTGAAACCAGAGAACG | NM001039605.1 |
| Reverse | ACAGAGGAGGGCATAGAGGATG | ||
| SIRT1 | Forward | GATCAGCAAAAGGCTGGATGGT | NM_001004767 |
| Reverse | ACGAGCCGCTTTCGCTACTAC | ||
| CPT-1 | Forward | ACAGCGAATGAAAGCAGGGT | NM_001012898.1 |
| Reverse | GCCATGGCTAAGGTTTTCGT | ||
| PPARα | Forward | AGTAAGCTCTCAGAAACTTTGTTG | NM_001001464.1 |
| Reverse | AAGGTTGAAACAGAAGCCGC | ||
| APOA1 | Forward | CGCATTCGGGATATGGTGGA | NM_205525 |
| Reverse | GTCAAGCTGTTTGCCCACAG | ||
| SREBP-1 | Forward | GACCATCCTGGCCACAGTAC | AF278697 |
| Reverse | TCTCAATGGCATTGTGGGCT | ||
| FAS | Forward | AGAGGCTTTGAAGCTCGGAC | NM_205155.2 |
| Reverse | GGTGCCTGAATACTTGGGCT | ||
| ACC | Forward | TTGTGGCACAGAAGAGGGAA | NM_205505.1 |
| Reverse | GTTGGCACATGGAATGGCAG | ||
| ACTB | Forward | TGCTGTGTTCCCATCTATCG | NM_205518.1 |
| Reverse | TTGGTGACAATACCGTGTTCA | ||
1
Abbreviations: ACC, acetyl-CoA carboxylase; ACTB, beta-actin; AMPKα, adenosine monophosphate–activated protein kinase alpha; ApoA1, apolipoprotein A1; CAT, catalase; SIRT1, silent 1; CPT-1, carnitine palmitoyltransferase 1; FAS, fatty acid synthase; GPX, glutathione peroxidase; HO-1, heme oxygenase-1; NRF2, nuclear factor erythroid 2–related factor 2; PPARα, peroxisome proliferator-activated receptor α; SOD1, superoxide dismutase 1; SREBP-1, sterol regulatory element-binding protein-1.