. 2020 Aug 7;99(11):5736–5743. doi: 10.1016/j.psj.2020.07.029

Table 3.

Effects of in ovo application of epidermal growth factor (EGF) on expression of EGF receptor from day 17 (E17) of incubation through to hatching (E21).

Incubation, day	EGF¹, μg	Relative expression
E17	0	1.02^b
E17	160	1.91^b
E17	640	1.43^b
E18	0	1.01^b
E18	160	0.90^b
E18	640	0.54^b
E19	0	1.06^b
E19	160	1.38^b
E19	640	1.62^b
E20	0	1.00^b
E20	160	1.87^b
E20	640	0.91^b
E21	0	1.07^b
E21	160	4.43^a
E21	640	5.64^a
SEM		0.517
Main effect of incubation day
E17		1.46^b
E18		0.82^b
E19		1.35^b
E20		1.26^b
E21		3.71^a
SEM		0.299
Main effect of EGF¹, μg
0		1.03^b
160		2.10^a
640		2.03^a
SEM		0.23
P value
Sampling day		<0.01
EGF		0.01
Sampling∗EGF		0.002

Means assigned different letters (a, b) within a response criterion are significantly different, P < 0.05; n = 5.

Epidermal growth factor, μg/kg egg, was injected into amniotic sac of eggs on day 19 of incubation.