Table 1.
Details of primers used for RT-qPCR.
| Linked pathway | Gene symbol (Accession no.) | Primer sequences (5' → 3′) | PCR product (bp) | Ta (°C) | References | |
|---|---|---|---|---|---|---|
| Lipid metabolism | FABP2 (NM_001007923) | F R |
TGGCATTTAACGGTACTTGGA TCAGATTATCGTGGGCTCCT |
111 | 60 | (Larkina et al., 2011) |
| CYP7A1 (NM_001001753.1) | F R |
CATTCTGTTGCCAGGTGATGTT GCTCTCTCTGTTTCCCGCTTT |
106 | 62 | (Wu et al., 2012) | |
| CYP27A1 (XM_422056.6) | F R |
AGGACTTTCGTCTGGCTCT CTCCGCATCGGGTATTT |
184 | 60 | (Hu et al., 2017) | |
| Drug metabolism | CYP1A4 (NM_205147) | F R |
TAAGGACGTCAATGCTCGTTTC CGTCCCGAATGTGCTCCTTAT |
88 | 62 | (Hickey et al., 2009) |
| GSTT1 (NM_205365.1) | F R |
CGGAGACTTCACCCTAGCAG GAGCATTAGCCCGGATGTTA |
155 | 60 | This study | |
| Reference gene | beta-actin (L08165.1) | F R |
ATGAAGCCCAGAGCAAAAGA GGGGTGTTGAAGGTCTCAAA |
223 | 60 | This study |
Abbreviations: F, forward; R, reverse; Ta, annealing temperature.