Table 2.

Diet formulation and calculated nutrient composition for laying period (18–95 wk).¹

Ingredients, %	Peaking2	Layer 2	Layer 3	Layer 4	Layer 5
Corn	53.61	62.99	61.54	64.18	62.57
Soybean meal	28.10	21.35	19.99	17.77	17.90
Soybean oil	3.75	2.90	3.00	2.87	3.21
Limestone	7.44	6.89	6.87	7.13	7.33
Oyster shell	3.19	2.95	2.94	3.06	3.14
Defluorinated phosphate	2.55	2.09	1.89	1.52	1.47
NaCl	0.30	0.30	0.30	0.30	0.30
L-Lysine HCl	0.46	0.09	0.04	0.05	0.04
DL-Methionine	0.33	0.22	0.17	0.14	0.14
Threonine	0.11	0.06	0.03	0.03	0.02
Vitamin premix3	0.05	0.05	0.05	0.05	0.05
Mineral premix4	0.06	0.06	0.06	0.06	0.06
Sand	0.05	0.05	3.11	2.84	3.78
Calculated value5
ME (kcal/kg)	2,840	2,900	2,820	2,840	2,820
CP%	19.05	16.15	15.27	14.42	14.32
Ca%	4.94	4.48	4.40	4.42	4.51
Available P (%)	0.58	0.49	0.45	0.38	0.37

Abbreviations: 5 ME, metabolic enenrgy; CP%, crude protein percentage; Ca, calcium percentage; Available P, available phosphorus percentage.

¹Treatments were added as the form of vitamin premix in the diet: D treatment, vitamin D₃ at 2,760 IU/kg; DD treatment, vitamin D₃ at 5,220 IU/kg; 25D treatment, vitamin D₃ at 2,760 IU/kg plus 25OHD at 2,760 IU (69 μg)/kg.

²Peaking (18–38 wk), layer 2 (39–48 wk), layer 3 (49–60 wk), layer 4 (61–75 wk), and layer 5 (76–95 wk).

³Supplied per kilogram of diet: vitamin A, 9,900 IU; vitamin E, 22.10 IU; vitamin B12, 0.02 mg; biotin, 0.06 mg; menadione, 3.3 mg; thiamine, 2.20 mg; riboflavin, 6.60 mg; pantothenic Acid, 11.00 mg; vitamin B6, 4.40 mg; niacin, 33.00 mg; folic acid, 0.90 mg; choline, 191.36 mg.

⁴Supplied per kilogram of diet: Mn, 80.4 mg; Zn, 64.2 mg; Mg, 16.08 mg; Fe, 15.78; Cu, 2.4 mg; I, 0.6 mg; Se, 0.24 mg.