Table 1.
Primers used for real-time PCR analysis of yolk sac tissue gene expression.
| Target1 | Accession no. | Primer F (5′-3′) | Primer R (5′-3′) | Ref. |
|---|---|---|---|---|
| LRP2 | XM_025152621 | GGAGACTCTGCTATAGACAGAGC | CCACACTACCAGCTCCTGTTA | Bauer et al., 2013 |
| ApoA1 | NM_205525.4 | GACCGCATTCGGGATATGGT | GGCCGGATCTTCTCCTTCAC | |
| PepT1 | NM_204365.1 | AAAACAGGTTTCGGCATCGC | CATCTCGATCCCTGCTGGTC | Yadgary and Uni, 2012 |
| FBP1 | NM_001278048.1 | TTCCATTGGGACCATATTTGG | ACCCGCTGCCACAAGATTAC | |
| GYS2 | XM_015291547.2 | CATCTGTACACTGTGCCCATGTG | TTTGGAGTGACAACATCAGGATTT | |
| TTR | NM_001281498.1 | CTCTTCTTGTTTTCTTAGCTGGAC | GACTGCATCCAGCACTTTCAC | Too et al., 2017 |
| DIO1 | NM_001097614.1 | TCAGGGTGCTCCTACACAAAC | TTTCGCCCAGCTTTAGGATGT | |
| DIO2 | NM_204114.4 | CGCCTACAAGCAGGTCAAA | ACTTGGTTCCACACTTGCCA | |
| β-Actin | NM_205518.1 | AATGGCTCCGGTATGTGCAA | GGCCCATACCAACCATCACA |
1
LRP2, LDL receptor; ApoA1, the major protein component of HDL; PepT1, oligopeptides transporter; FBP1, gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose; GYS2, catalyzes the rate-limiting step in the synthesis of glycogen; TTR, THs carrier protein in the plasma; DIO1, THs activating and deactivating enzyme; DIO2, THs activating enzyme; β-Actin, housekeeping gene.