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Method for measuring villus height (VH) and crypt depth (CD). Formalin-fixed, paraffin embedded tissue sections were processed by in situ hybridization to identify stem cells in the crypt that expressed olfactomedin 4 (Olfm4) mRNA (brown staining). ImageJ software was used to measure the functional CD (red line) and VH (yellow line). Image was taken at 40× magnification. The scale bar represents 0.1 mm.
