Figure 6.

Effect of 36 h delayed access to feed on cells expressing Muc2 mRNA in the small intestine. Formalin-fixed, paraffin embedded tissues from the duodenum (Duo), jejunum (Jej), and ileum (Ile) were sectioned and stained for Muc2 mRNA by in situ hybridization (n = 3). Images are from control chicks with no feed delay (ND), chicks with 24 h feed delay plus 12 h of feeding (DAF24) and chicks with 36 h feed delay (DAF36) sampled at 36 h after hatch. Brown staining represents Muc2 mRNA expression in cells. Tissues were counterstained with hematoxylin. Images were taken at 40x magnification. Scale bar represents 0.1 mm.